9YNZ

Human PU.1 ETS-Domain (165-270) Bound to d(5'-AATAAGCGGAAGTGGG-3') d(5'-TCCCACT*CPD*CGCTTAT-3')

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920100
Spacegroup name	P 1 21 1
Unit cell lengths	42.966, 58.879, 45.925
Unit cell angles	90.00, 117.59, 90.00

Refinement procedure

Resolution	23.850 - 2.050
R-factor	0.2103
Rwork	0.206
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.635
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.850	2.123
High resolution limit [Å]	2.050	2.050
Rmerge	0.105	0.429
Rmeas	0.122	0.496
Rpim	0.061	0.246
Number of reflections	12776	1256
<I/σ(I)>	7.43	1.92
Completeness [%]	99.0	99.52
Redundancy	3.7	3.9
CC(1/2)	0.993	0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	2% PEG 3350, 100 mM Sodium Acetate, pH 4.6