9YKF
Crystal Structure of E. coli D23N YajL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.88557 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.723, 78.335, 99.614 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.170 - 0.980 |
| R-factor | 0.0974 |
| Rwork | 0.097 |
| R-free | 0.11440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.137 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.040 | 1.000 |
| High resolution limit [Å] | 0.980 | 0.980 |
| Rmeas | 0.048 | 0.790 |
| Number of reflections | 194272 | 8354 |
| <I/σ(I)> | 20.5 | |
| Completeness [%] | 99.0 | |
| Redundancy | 7.7 | |
| CC(1/2) | 1.000 | 0.754 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 22-25% PEG 4000, 200 mM MgCl2, and 100 mM Tris, pH=8.5 |
