9YK3
Room-temperature X-ray structure of D132N Bacillus halodurans RNase H1 in complex with complementary RNA/DNA duplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2025-06-10 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.974, 37.935, 62.155 |
| Unit cell angles | 90.00, 96.11, 90.00 |
Refinement procedure
| Resolution | 21.400 - 1.900 |
| R-factor | 0.2025 |
| Rwork | 0.201 |
| R-free | 0.23110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.676 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.800 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.112 | 0.563 |
| Rpim | 0.064 | 0.366 |
| Number of reflections | 14414 | 1377 |
| <I/σ(I)> | 16.2 | 1.8 |
| Completeness [%] | 94.7 | 90.1 |
| Redundancy | 4.3 | 3.3 |
| CC(1/2) | 0.950 | 0.538 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES pH 7.5, 0.2 M NaCl, and 12-16% PEG 10,000 |
