9YJ4

TGM6-D3 bound to mouse TBRII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92010
Spacegroup name	P 21 21 21
Unit cell lengths	53.353, 92.589, 93.393
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.880 - 2.520
R-factor	0.2599
Rwork	0.259
R-free	0.28800
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.880	2.776
High resolution limit [Å]	2.517	2.517
Rmerge		2.066
Rmeas	0.257	2.171
Rpim	0.089	0.659
Number of reflections	12574	629
<I/σ(I)>	6.5	1.4
Completeness [%]	92.2	42
Redundancy	8	10.5
CC(1/2)	0.991	0.369

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289.15	0.1M Sodium HEPES pH 7.5, 10% PEG 4000, 0.1M MgCl2