9YIU

Macrophage Migration Inhibitory Factor 2 from Necator americanus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979338
Spacegroup name	H 3 2
Unit cell lengths	64.653, 64.653, 174.687
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.115 - 1.726
Rwork	0.191
R-free	0.20880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.731
Data reduction software	XDS (Jun 30, 2023 (BUILT 20230630))
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.115	1.756
High resolution limit [Å]	1.726	1.726
Rmerge	0.052	1.685
Rmeas	0.057	1.861
Rpim	0.024	0.774
Number of reflections	14008	737
<I/σ(I)>	12.7	0.8
Completeness [%]	92.3	100
Redundancy	5.71	5.56
CC(1/2)	0.999	0.446

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	1.26 M NaH2PO4 (Precipitant), 0.14 M K2HPO4 (Precipitant)