9YGX
I21T mutant human DJ-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.729 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 74.962, 74.962, 75.463 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.480 - 0.970 |
| R-factor | 0.0998 |
| Rwork | 0.099 |
| R-free | 0.11270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.154 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.730 | 0.990 |
| High resolution limit [Å] | 0.970 | 0.970 |
| Number of reflections | 144677 | 7037 |
| <I/σ(I)> | 16.4 | |
| Completeness [%] | 99.9 | |
| Redundancy | 11.1 | |
| CC(1/2) | 0.999 | 0.394 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 22-27% PEG4000, 0.2 M sodium acetate, and 100 mM Tris HCl, pH=7.4 |
