9YFX

KrKA retaining Kdo transferase CMP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.95358
Spacegroup name	P 43 21 2
Unit cell lengths	132.850, 132.850, 197.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.970 - 2.700
R-factor	0.2088
Rwork	0.208
R-free	0.22910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.633
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.970	2.730
High resolution limit [Å]	2.700	2.700
Rmeas		5.014
Number of reflections	131580	1094
<I/σ(I)>	6.3
Completeness [%]	99.9
Redundancy	25.2
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2M potassium chloride, 0.1 M glycine pH 9.5 and 20% v/v pentaerythritol ethoxylate (15/4 EO/OH)