9YDY

Context-Dependent Variability Of HIF Heterodimers Influences Interactions With Macromolecular And Small Molecule Partners

Replaces:  9OPF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-05
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9201
Spacegroup name	C 1 2 1
Unit cell lengths	263.079, 23.827, 31.856
Unit cell angles	90.00, 91.81, 90.00

Refinement procedure

Resolution	32.870 - 2.300
R-factor	0.2511
Rwork	0.250
R-free	0.26370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.413
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	131.474	2.296
High resolution limit [Å]	2.178	2.178
Number of reflections	9098	456
<I/σ(I)>	4.4
Completeness [%]	87.4
Redundancy	3.1
CC(1/2)	0.992	0.660

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.1 M imidazole, 50% (+/-)-2-Methyl-2,4-pentanediol (MPD)