9YDF

Structure of the cyclic nucleotide binding domain of SLC9C1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-12-11
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97648
Spacegroup name	P 61 2 2
Unit cell lengths	45.788, 45.788, 276.932
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.160 - 2.180
R-factor	0.20423
Rwork	0.203
R-free	0.23745
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.461
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.220
High resolution limit [Å]	2.180	5.910	2.180
Rmerge	0.085	0.053	0.974
Rmeas	0.087	0.054	0.994
Rpim	0.016	0.011	0.194
Total number of observations	286680
Number of reflections	9891	638	431
<I/σ(I)>	6.3
Completeness [%]	99.4	99.8	95.1
Redundancy	29	23.3	24.8
CC(1/2)	0.999	0.999	0.892

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	20% (w/v) polyethylene glycol (PEG) 3350, 0.2 M Calcium chloride dihydrate