9YD4
RNA primer non-enzymatic extension product with DNA substrate
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 99 |
| Detector technology | PIXEL |
| Collection date | 2022-06-23 |
| Detector | DECTRIS EIGER2 X CdTe 16M |
| Wavelength(s) | 1.033175 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 48.254, 48.254, 82.532 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.282 - 1.658 |
| Rwork | 0.257 |
| R-free | 0.28910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.575 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.690 |
| High resolution limit [Å] | 1.658 | 1.658 |
| Rmerge | 0.115 | 0.287 |
| Rmeas | 0.124 | 0.311 |
| Rpim | 0.044 | 0.116 |
| Number of reflections | 13714 | 646 |
| <I/σ(I)> | 22.3 | 8 |
| Completeness [%] | 99.6 | 96.9 |
| Redundancy | 8.7 | 6.1 |
| CC(1/2) | 0.963 | 0.992 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.05 M HEPES pH 7.0, 0.2 M Ammonium acetate, 0.15 M Magnesium acetate, 10% w/v Polyethylene glycol 6,000. |
