9YD3

RNA primer non-enzymatic extension product with RNA substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	99
Detector technology	PIXEL
Collection date	2022-04-23
Detector	DECTRIS EIGER2 X CdTe 16M
Wavelength(s)	1.033175
Spacegroup name	P 3 2 1
Unit cell lengths	47.338, 47.338, 83.136
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.568 - 1.592
Rwork	0.226
R-free	0.24740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.022
RMSD bond angle	2.968
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.630
High resolution limit [Å]	1.592	1.592
Rmerge	0.058	0.336
Rmeas	0.062	0.366
Rpim	0.022	0.139
Number of reflections	14954	650
<I/σ(I)>	37.2	5.5
Completeness [%]	99.3	88.8
Redundancy	8.4	5.7
CC(1/2)	0.982	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.05 M HEPES pH 7.0, 0.2 M Ammonium acetate, 0.15 M Magnesium acetate, 10% w/v Polyethylene glycol 6,000