9YCU
Protiated human DJ-1, 100K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.729 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 75.109, 75.109, 75.505 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.550 - 1.050 |
| R-factor | 0.1187 |
| Rwork | 0.118 |
| R-free | 0.13420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.083 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.750 | 1.070 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmeas | 0.075 | 3.838 |
| Number of reflections | 114819 | 5635 |
| <I/σ(I)> | 13.2 | 0.6 |
| Completeness [%] | 100.0 | 99.6 |
| Redundancy | 10.6 | 10.2 |
| CC(1/2) | 0.999 | 0.252 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 22-24% PEG 3000, 200 mM NaCl, and 100 mM HEPES, pH 7.5 |
