9YBZ

CRYSTAL STRUCTURE OF THE A149T VARIANT OF SERINE HYDROXYMETHYLTRANSFERASE 8 FROM SOYBEAN CULTIVAR ESSEX IN COMPLEX WITH PLP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2025-05-24
Detector	RDI CMOS_8M
Wavelength(s)	1.000
Spacegroup name	P 31 2 1
Unit cell lengths	174.318, 174.318, 184.208
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.540 - 2.280
R-factor	0.2742
Rwork	0.272
R-free	0.31280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.075
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.540	2.320
High resolution limit [Å]	2.280	2.280
Rmerge	0.182	1.792
Rmeas	0.194	1.902
Rpim	0.065	0.630
Number of reflections	145304	146610
<I/σ(I)>	11.4	1.5
Completeness [%]	99.8	99.9
Redundancy	8.8	9
CC(1/2)	0.998	0.599

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Ammonium tartrate dibasic pH 7.0 12% w/v Polyethylene glycol 3,350