9YBR

Binding Sites in the ARID1B DNA Binding Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-08-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.953725
Spacegroup name	P 65
Unit cell lengths	68.835, 68.835, 46.006
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.420 - 1.450
R-factor	0.1515
Rwork	0.151
R-free	0.16100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.012
Data reduction software	autoPROC
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.420	1.500
High resolution limit [Å]	1.450	1.450
Rmerge	0.090	1.503
Rpim	0.020
Number of reflections	22134	2198
<I/σ(I)>	18.6	2.1
Completeness [%]	100.0	100
Redundancy	20.35	19.11
CC(1/2)	0.999	0.814

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	1.5 M sodium citrate, pH 6.5, 0.033% w/v 4-nitrobenzoic acid, 0.033% w/v 5-sulfosalicylic acid dihydrate, 0.033% w/v naphthalene-1,3,6-trisulfonic acid trisodium salt hydrate, 0.002 M HEPES sodium, pH 6.8