9YBG

Anti HPA-1a 26.4Fab and integrin beta 3 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97946
Spacegroup name	C 2 2 21
Unit cell lengths	84.340, 176.070, 178.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.900 - 2.290
R-factor	0.186
Rwork	0.184
R-free	0.23240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.914
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.950	2.420
High resolution limit [Å]	2.290	2.290
Number of reflections	60089	5936
<I/σ(I)>	5.4
Completeness [%]	99.7
Redundancy	5.4
CC(1/2)	0.966	0.779

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292.15	sodium citrate, PEG 4000.