9YB6

Human Mitochondrial Aspartate Aminotransferase complex with PLP at 1.5 Angstrom.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1
Unit cell lengths	57.180, 59.168, 67.167
Unit cell angles	101.39, 101.72, 95.41

Refinement procedure

Resolution	48.515 - 1.500
Rwork	0.185
R-free	0.20940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.896
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.515	1.530
High resolution limit [Å]	1.500	1.500
Number of reflections	129693	23783
<I/σ(I)>	6.1	0.7
Completeness [%]	96.8
Redundancy	3.7
CC(1/2)	0.995	0.361

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1 M Ammonium Sulfate, 0.05 M Sodium Acetate, 23% PEG-3350, pH 5.5