9YAK

Structure of the GCN2 pseudokinase domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 2.0.1
Synchrotron site	ALS
Beamline	2.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0387
Spacegroup name	P 4 2 2
Unit cell lengths	118.975, 118.975, 62.673
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.060 - 2.980
R-factor	0.2692
Rwork	0.265
R-free	0.30400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.135
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.060	3.086
High resolution limit [Å]	2.980	2.980
Number of reflections	9609	922
<I/σ(I)>	15
Completeness [%]	99.2
Redundancy	12.8
CC(1/2)	0.999	0.283

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	100 mM MES pH 6.5 and 1.6 M magnesium sulfate