9YA5
KIF1A R350G bound to microtubules in two-heads-bound state with AMP-PNP
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 0.001 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 2.95 |
| Software | cryoSPARC,PHENIX,UCSF Chimera,Rosetta |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
