9Y8V
Crystal structure of the Kelch domain of human KLHL12 with compound 10q
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-03 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 79.571, 79.571, 76.631 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.329 - 1.271 |
| Rwork | 0.160 |
| R-free | 0.19940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.700 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.329 | 1.380 |
| High resolution limit [Å] | 1.271 | 1.271 |
| Rmerge | 0.051 | 1.060 |
| Number of reflections | 59437 | 1291 |
| <I/σ(I)> | 25.2 | 0.7 |
| Completeness [%] | 96.4 | 50.4 |
| Redundancy | 10.7 | 1.7 |
| CC(1/2) | 1.000 | 0.404 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M Bis-Tris pH 5.5, 0.2M MgCl2 and 25% PEG3350 |
