9Y4V
Crystal structure of a GH5_18 from Microbacterium oxydans DSM 20578
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-04 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.237, 95.142, 102.056 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.570 - 1.920 |
| R-factor | 0.1726 |
| Rwork | 0.170 |
| R-free | 0.21580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.785 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.570 | 1.970 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rpim | 0.043 | 0.163 |
| Number of reflections | 60511 | 3907 |
| <I/σ(I)> | 16.2 | |
| Completeness [%] | 99.3 | 96.7 |
| Redundancy | 5.3 | 4.7 |
| CC(1/2) | 0.998 | 0.847 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% (w/v) PEG 3350, 0.2 M MgCl2, 0.1 M Tris pH 8.5 |
