9Y47
Human nucleosome structure on Nickel-NTA lipid affinity grid (C1 refinement)
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS TALOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.74 |
| Software | RELION,PHENIX,SerialEM,UCSF Chimera |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
