9Y3Y
Crystal structure of hemagglutinin from H1N1 Influenza A virus A/California/04/2009 bound to the 49_C09 antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-08-16 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 166.919, 166.919, 347.947 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.710 - 3.800 |
| R-factor | 0.2067 |
| Rwork | 0.205 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.943 |
| Data reduction software | HKL-2000 (v722) |
| Data scaling software | HKL-2000 (v722) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.870 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rpim | 0.150 | |
| Number of reflections | 43492 | 1669 |
| <I/σ(I)> | 5.4 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.9 | |
| CC(1/2) | 1.000 | 0.550 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 2M ammonium sulfate, 15% (v/v) ethylene glycol, 0.2M lithium sulfate, 0.1M CAPS, pH 10.5 |
