9Y0R
Importin alpha 2 in complex with ATF2 basic region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-14 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.611, 90.120, 100.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.620 - 2.200 |
| R-factor | 0.1928 |
| Rwork | 0.191 |
| R-free | 0.22160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.503 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.620 | 2.700 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rpim | 0.071 | 0.799 |
| Number of reflections | 37013 | 16439 |
| <I/σ(I)> | 7.6 | 2.2 |
| Completeness [%] | 99.9 | |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296 | sodium citrate 0.7 M, 0.1 M HEPES, 10 mM DTT, pH 7 |
