9Y01
QatB-QatC complex in qatABCD anti-phage defense
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-05-24 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.919901 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.871, 74.590, 133.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.250 - 1.310 |
| R-factor | 0.1841 |
| Rwork | 0.183 |
| R-free | 0.20320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.125 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.250 | 1.320 |
| High resolution limit [Å] | 1.307 | 1.310 |
| Rmerge | 0.082 | 2.069 |
| Rmeas | 0.087 | 2.221 |
| Rpim | 0.030 | 0.798 |
| Number of reflections | 134772 | 4405 |
| <I/σ(I)> | 10.43 | 1.08 |
| Completeness [%] | 76.4 | 0.29 |
| Redundancy | 8.5 | 7.7 |
| CC(1/2) | 0.999 | 0.303 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291.15 | 0.1 M NaCl, 18% PEG-3350 |
