9XY6

One of a series of engineered variants of I-OnuI meganuclease targeting altered DNA target sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	108
Detector technology	CCD
Collection date	2018-03-29
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	43.185, 64.283, 167.825
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.398 - 2.693
R-factor	0.2445
Rwork	0.239
R-free	0.29350
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.8.1)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.800
High resolution limit [Å]	2.693	5.810	2.700
Rmerge	0.072	0.039	0.387
Rmeas	0.075	0.041	0.410
Rpim	0.021	0.012	0.128
Number of reflections	12932	1494	968
<I/σ(I)>	12.1
Completeness [%]	95.0	99.5	73.8
Redundancy	12.5	12.4	9.4
CC(1/2)		0.999	0.963

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.25M DL-Malic acid pH 7.0, 35% PEG 3350, 100mM Tris pH 7.0