9XLU

Crystal structure of Staphylococcus aureus cystathionine gamma-lyase V129G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-10-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	I 2 2 2
Unit cell lengths	62.832, 80.467, 161.067
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.270 - 2.330
R-factor	0.2123
Rwork	0.207
R-free	0.26030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.583
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.21.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.270	2.410
High resolution limit [Å]	2.330	2.330
Rmerge	0.083
Rmeas	0.089
Rpim	0.031
Number of reflections	25074	858
<I/σ(I)>	17.5	4.4
Completeness [%]	87.2
Redundancy	1.01	1
CC(1/2)	0.997	0.962

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287.15	16% PEG 3350, 1.5% tryptone, 0.05M HEPES sodium pH 7.0, 0.01M sodium azide