Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2021-12-30 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 32.735, 157.955, 27.795 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.050 - 2.100 |
| R-factor | 0.2179 |
| Rwork | 0.213 |
| R-free | 0.25920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.991 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: 000)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.050 | 2.170 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Number of reflections | 8960 | 8957 |
| <I/σ(I)> | 24.6 | |
| Completeness [%] | 99.0 | |
| Redundancy | 12.1 | |
| CC(1/2) | 0.996 | 0.868 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | PEG 8000 20.5% PEG 400 20% 100mM MgCl2 100mM pH 7.0 Tris-HCl |
