Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2022-04-21 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.137, 63.968, 77.145 |
| Unit cell angles | 90.00, 93.95, 90.00 |
Refinement procedure
| Resolution | 32.970 - 2.280 |
| R-factor | 0.2027 |
| Rwork | 0.200 |
| R-free | 0.25510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.975 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.970 | 2.360 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Number of reflections | 14764 | 14388 |
| <I/σ(I)> | 26.6 | |
| Completeness [%] | 99.7 | |
| Redundancy | 7.5 | |
| CC(1/2) | 0.996 | 0.944 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | PEG 400, Magnesium chloride, pH 7.5 HEPES, Sodium Thiocyanate |
