9XB8
ATP-dependent diazotase Mco01_40450
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2024-07-06 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.01300 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.193, 163.993, 107.686 |
| Unit cell angles | 90.00, 99.71, 90.00 |
Refinement procedure
| Resolution | 49.010 - 3.400 |
| R-factor | 0.1602 |
| Rwork | 0.154 |
| R-free | 0.23710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.067 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.010 | 3.610 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Number of reflections | 28803 | 4632 |
| <I/σ(I)> | 8.3 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 7.4 |
| CC(1/2) | 0.974 | 0.754 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% (w/v) PEG 8000, 100 mM imidazole/hydrochloric acid (pH6.5), and 3% (v/v) MPD 2-methyl-2,4-pentanediol |
