9X8F

Crystal structure of the N-terminal of an alpha-amylase family glycosyl hydrolase from Vibrio parahaemolyticus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-10-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 41 21 2
Unit cell lengths	118.770, 118.770, 107.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.000 - 1.960
R-factor	0.20093
Rwork	0.199
R-free	0.24630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.571
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.060	2.010
High resolution limit [Å]	1.960	1.960
Number of reflections	55878	4057
<I/σ(I)>	6
Completeness [%]	99.9
Redundancy	24.2
CC(1/2)	0.989	0.327

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	0.2 M Ammonium sulfate, 25% w/v Polyethylene glycol 3,350, 0.1 M BIS-TRIS pH 5.5.