9X7M
core filament of the spirochete periplasmic flagella of Leptospira biflexa from the deleted fcpB_CL13 strain
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 0.01 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.2 |
| Software | cryoSPARC,SerialEM,Coot,PHENIX |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING ONLY |
