9X7K
core filament of the spirochete periplasmic flagella of Leptospira biflexa wild type
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 0.01 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.35 |
| Software | cryoSPARC,PHENIX,EPU,Coot |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING ONLY |
