9X77
X-ray structure of Paenibacillus kribbensis D-allose kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 79.370, 79.370, 117.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.270 - 1.580 |
| R-factor | 0.17985 |
| Rwork | 0.179 |
| R-free | 0.19894 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.911 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0431) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.270 | 1.620 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.053 | |
| Number of reflections | 52157 | 3787 |
| <I/σ(I)> | 27.5 | |
| Completeness [%] | 99.9 | |
| Redundancy | 12.4 | |
| CC(1/2) | 0.999 | 0.830 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM Ammonium tartrate dibasic pH6.6, 20% w/v Polyethylene glycol 3,350 |
