9X6Q

Crystal structure of the Songling virus nucleoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 2 21 21
Unit cell lengths	54.490, 113.352, 215.687
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.270 - 2.520
R-factor	0.2141
Rwork	0.212
R-free	0.25720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.109
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.680	2.610
High resolution limit [Å]	2.520	2.520
Number of reflections	45915	4370
<I/σ(I)>	9.32
Completeness [%]	99.8	98.5
Redundancy	12.5	13
CC(1/2)	0.996	0.635

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	90 mM LiNaK (0.3 M Lithium sulfate, 0.3 M Sodium sulfate, 0.3 M Potassium sulfate); 0.1 M Buffer System 4 (1.0M MOPSO, Bis-Tris, PH 6.5); PH 6.5; 50 % v/v Precipitant Mix 6 (25% w/v PEG 4000, 40% w/v 1,2,6- Hexanetriol)