9X43
Crystal structure of glycosyltransferase UGT73C1 in complex with UDP and quercetin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-03-30 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 85.465, 88.253, 132.045 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.395 - 2.081 |
| R-factor | 0.2108 |
| Rwork | 0.208 |
| R-free | 0.25370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.586 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.16_3549: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.370 | 2.190 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.287 | 2.455 |
| Rmeas | 0.304 | 2.629 |
| Rpim | 0.099 | 0.930 |
| Number of reflections | 29720 | 4392 |
| <I/σ(I)> | 3.4 | 0.8 |
| Completeness [%] | 97.9 | 100 |
| Redundancy | 8.5 | 7.3 |
| CC(1/2) | 0.985 | 0.519 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291.15 | 0.1M Bis-Tris pH 6.5, 25% PEG3350 |
