9X3M

Crystal structure of Pseudopedobacter saltans GH43 beta-xylosidase in complex with xylose.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-29
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.87313
Spacegroup name	P 1
Unit cell lengths	73.585, 89.435, 138.443
Unit cell angles	102.82, 94.02, 97.88

Refinement procedure

Resolution	47.841 - 2.800
Rwork	0.218
R-free	0.27280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8hcj
RMSD bond length	0.011
RMSD bond angle	2.317
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.100))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.842	2.870
High resolution limit [Å]	2.800	2.800
Rmerge	0.170	0.370
Rmeas	0.240	0.520
Number of reflections	78398	73759
<I/σ(I)>	4	2
Completeness [%]	93.0	94
Redundancy	1.8	1.8
CC(1/2)	0.900	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293.15	5% propanol, 0.1M imidazole and 14% PEG 3550.