9WYS

Structural basis of signal activation and transduction by chitin elicitor receptor kinase 1 in Oryza sativa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-09-14
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	P 31 2 1
Unit cell lengths	109.910, 109.910, 120.140
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.913 - 2.790
R-factor	0.2315
Rwork	0.228
R-free	0.26380
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.913	2.860
High resolution limit [Å]	2.790	2.790
Number of reflections	21356	1546
<I/σ(I)>	14.1
Completeness [%]	100.0
Redundancy	19.8
CC(1/2)	0.998	0.681

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.04 M HEPES sodium pH 7.5, 4% v/v 2-Propanol, 0.02 M Sodium citrate tribasic dihydrate, 2% w/v Polyethylene glycol 3,350, 8% w/v Polyethylene glycol 4,000