9WYR

Structural basis of signal activation and transduction by chitin elicitor receptor kinase 1 in Oryza sativa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-09-14
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	P 31 2 1
Unit cell lengths	108.757, 108.757, 121.457
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	74.429 - 2.552
R-factor	0.2372
Rwork	0.234
R-free	0.26960
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.190	2.690
High resolution limit [Å]	2.550	2.550
Number of reflections	26159	3959
<I/σ(I)>	12.3
Completeness [%]	95.0
Redundancy	9.3
CC(1/2)	0.995	0.446

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	(0.009 M Citric, 0.02M B-T propane) pH 7.1, 1.1% v/v TacsimateTM pH 7.0, 0.007 M HEPES pH 7.0, 0.009 M HEPES pH 7.5, 1.8% v/v Jeffamine M-600, 0.09 M Lithium chloride, 7.5% w/v Polyethylene glycol 3,350