9WYD

Crystal structure of HAstV8 spike and FcRn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-13
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	C 1 2 1
Unit cell lengths	173.120, 84.429, 53.953
Unit cell angles	90.00, 97.42, 90.00

Refinement procedure

Resolution	37.880 - 2.650
R-factor	0.1893
Rwork	0.187
R-free	0.23230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.541
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.810
High resolution limit [Å]	2.650	2.650
Rpim		0.090
Number of reflections	104873	22569
<I/σ(I)>	9.07
Completeness [%]	99.7
Redundancy	4.64
CC(1/2)	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		291.15	0.1 M BICINE pH 8.5, 8% w/v Polyethylene glycol monomethyl ether 5,000