9WY3

Crystal structure of Frog M-ferritin E130A_L165D mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	103
Detector technology	IMAGE PLATE
Collection date	2015-01-24
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	F 4 3 2
Unit cell lengths	183.760, 183.760, 183.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.390 - 1.770
Rwork	0.157
R-free	0.18800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ka3
RMSD bond length	0.013
RMSD bond angle	1.449
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0431 (REFMACAT 0.4.105))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.390	1.810
High resolution limit [Å]	1.770	1.770
Number of reflections	26270	15500
<I/σ(I)>	22
Completeness [%]	100.0
Redundancy	11
CC(1/2)	0.999	0.967

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	277	2.0 M MgCl2 and 100 mM Bicine (pH 9.0)