9WXJ

Cryo-EM structure of the type III-D2 CRISPR-Cas effector complex bound to a non-cognate target RNA in the pre-cleavage state

EM specimen

Vitrification

Yes

EM Data Collection

Microscope	TFS KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Detector	GATAN K3 (6k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.7
Software	cryoSPARC,Servalcat
Symmetry	C1
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION