9WX2

Crystal structure of the HEPN domain of Dandelion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	61.476, 67.957, 74.935
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.738 - 1.548
R-factor	0.1765
Rwork	0.176
R-free	0.20110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.789
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.580
High resolution limit [Å]	1.548	4.210	1.550
Rmerge	0.097	0.073	1.009
Rmeas	0.101	0.076	1.085
Rpim	0.030	0.023	0.387
Number of reflections	46384	2520	2242
<I/σ(I)>	6.4
Completeness [%]	99.8	100	97.4
Redundancy	11.4	11.1	7
CC(1/2)	0.994	0.995	0.633

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	20% (w/v) PEG 6000, 0.2 M NaCl, and 0.1 M HEPES (pH 7.0)