9WWZ

Crystal structure of CTD of Dandelion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-01-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97861
Spacegroup name	P 1 21 1
Unit cell lengths	50.610, 150.880, 51.339
Unit cell angles	90.00, 90.71, 90.00

Refinement procedure

Resolution	50.606 - 2.785
R-factor	0.2343
Rwork	0.232
R-free	0.27050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.605
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.606	2.940
High resolution limit [Å]	2.785	2.790
Rpim	0.112	0.382
Number of reflections	19142	2794
<I/σ(I)>	7.7
Completeness [%]	99.4	100
Redundancy	6.7
CC(1/2)	0.984

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0% (w/v) PEG 8000, 0.05 M KCl, 0.05 M sodium cacodylate (pH 6.0), 0.5 mM spermine, and 0.5 mM L-argininamide dihydrochloride.