9WVV
The crystal structure of OspA mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-07 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.260, 54.990, 66.540 |
| Unit cell angles | 90.00, 100.42, 90.00 |
Refinement procedure
| Resolution | 19.850 - 1.270 |
| R-factor | 0.1957 |
| Rwork | 0.195 |
| R-free | 0.21620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.893 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.850 | 1.320 |
| High resolution limit [Å] | 1.270 | 1.270 |
| Rmerge | 0.032 | 0.111 |
| Number of reflections | 61196 | 5724 |
| <I/σ(I)> | 18.2 | |
| Completeness [%] | 99.2 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 40% PEG 400 (Hampton), 0.1M Tris-HCl (pH 8.0), 18.0mg/mL |
