9WVU
Carbohydrate-bound structure of alpha-glucan phosphorylase from Crocosphaera subtropica ATCC 51142
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 267.238, 267.238, 204.452 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.005 - 2.700 |
| Rwork | 0.174 |
| R-free | 0.22670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.667 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.500 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.074 | 0.360 |
| Rmeas | 0.083 | 0.406 |
| Rpim | 0.038 | 0.187 |
| Number of reflections | 149465 | 21830 |
| <I/σ(I)> | 8.2 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.9 | 4.7 |
| CC(1/2) | 0.799 | 0.762 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 45% (w/v) PEG 400, 0.1M Tris-HCl pH 8.0, 0.2M lithium sulfate |
