9WT9
Crystal structure of a multiheme cytochrome c selenoprotein mutant (MccSep U325A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-18 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.170, 122.650, 116.450 |
| Unit cell angles | 90.00, 92.15, 90.00 |
Refinement procedure
| Resolution | 48.530 - 1.800 |
| R-factor | 0.1887 |
| Rwork | 0.187 |
| R-free | 0.22420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.054 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.530 | 1.864 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.081 | |
| Number of reflections | 215383 | 21438 |
| <I/σ(I)> | 14.33 | |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 7 | |
| CC(1/2) | 0.999 | 0.601 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M ammonium sulfate, 0.1M HEPES (pH 7.5), 10% PEG 4000 |
