9WT3

NRBF2 coiled coil domain promotes autophagy by strengthening association with Vps15 in the PI3KC3 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	289
Detector technology	IMAGE PLATE
Collection date	2025-05-15
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	84.529, 132.810, 87.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.680 - 2.250
R-factor	0.21818
Rwork	0.216
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	in-house SeMet derivative model
RMSD bond length	0.007
RMSD bond angle	1.793
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	87.290	2.370
High resolution limit [Å]	2.250	2.250
Rmerge	0.051	0.148
Number of reflections	23604	3380
<I/σ(I)>	33.7
Completeness [%]	100.0	99.8
Redundancy	12.4	12
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	3.916 M NaCl and 0.1 M Tris buffer (pH 7.0)