9WS0

Crystal structure of cZ22-Fab in complex with left-handed d(CG)6 DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	112
Detector technology	CCD
Collection date	2024-06-06
Detector	RAYONIX MX300HE
Wavelength(s)	1.0
Spacegroup name	I 21 21 21
Unit cell lengths	114.849, 148.663, 172.179
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.330 - 3.270
R-factor	0.2005
Rwork	0.199
R-free	0.23850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.789
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.330	3.390
High resolution limit [Å]	3.270	3.270
Number of reflections	23145	2268
<I/σ(I)>	8.5
Completeness [%]	99.8
Redundancy	6.79
CC(1/2)	0.990	0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	30% (w/v) PEG 400, 0.2 M sodium citrate tribasic, and 0.1 M Tris-HCl, pH 8.5