9WP4

Crystal structure of phosphate binding protein from Synechocystis sp. PCC 6803

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	37.768, 64.843, 129.157
Unit cell angles	90.00, 91.39, 90.00

Refinement procedure

Resolution	37.760 - 1.410
R-factor	0.1792
Rwork	0.176
R-free	0.23570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.979
Phasing software	PHENIX ((1.20.1_4487: ???))
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.760	1.490
High resolution limit [Å]	1.410	1.410
Number of reflections	73938	833
<I/σ(I)>	8.4
Completeness [%]	61.7
Redundancy	2.6
CC(1/2)	0.988	0.789

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293.15	0.2M MgCl2, 0.1M Tris pH 9.0, 30% PEG 3000.