9WOZ

Crystal structure of the glycine oxidase from Bacillus subtilis with FAD and 2-(Methylthio)acetic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2024-09-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97923
Spacegroup name	P 61 2 2
Unit cell lengths	139.735, 139.735, 213.250
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.200 - 2.490
R-factor	0.2255
Rwork	0.223
R-free	0.26950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.525
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.490	2.500
Number of reflections	43397	2107
<I/σ(I)>	18
Completeness [%]	100.0
Redundancy	34.9
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.2 M Sodium malonate, pH 7.0, 22% w/v PEG 3350